Steroid adduct formation with lens crystallins

Diabetic cataract formation: potential role of glycosylation of lens crystallins.

A high glucose concentration in vivo or an increased glucose of glucose 6-phosphate concentration in vitro has been found to lead to the glycosylation of epsilon-amino groups of lysine residues in bovine and rat lens crystallins. In vitro, this glycosylation imparts an increased susceptibility of the crystallins to sulfhydryl oxidation. Disulfide crosslinks result in the formation of high molec...

Age-dependent association of γ-crystallins with aged α-crystallins from old bovine lens

PURPOSE Previous theoretical and experimental studies have predicted that the loss of weak protein interactions between alpha- and gamma-crystallins could result in a decrease in the transparent properties of the aging lens. METHODS alpha-Crystallins were prepared from the nucleus of old bovine lens, and gamma-crystallins were prepared from whole fetal bovine lens or from the nucleus of old b...

Subunit composition of rabbit lens beta crystallins.

DEAE-purified rabbit beta crystallins have been studied by sedimentation on sucrose gradients ami their subunits characterized by co-electrophoresis in SDS-polyacrylamide gels. The experiments indicate that the beta crystallins isolated by DEAE-cellulose are a large family of related proteins with sedimentation velocities of about 4S. Deaggregation and denaturation of these proteins to polypept...

Ontogeny of lens crystallins in marine cephalopods.

The ontogeny of the lens crystallin antigens throughout development of three marine cephalopod embryos, Loligo vulgaris, Sepia officinalis and Octopus vulgaris has been investigated using the indirect immuno-cytochemical staining against respective homologous anti-total lens protein antiserum. The cellular mechanism of lens development appears to be the same in all species investigated and so d...

Lens crystallins. Innovation associated with changes in gene regulation.